jijoye cell line  (ATCC)

Journal: Molecular Therapy. Nucleic Acids

Article Title: Extracellular viral microRNAs as biomarkers of virus infection in human cells

doi: 10.1016/j.omtn.2024.102444

Figure Lengend Snippet: Viral exmiRs identify viral reactivation in cells (A–C) RT-dPCR quantification of cellular EBV genes EBNA1 (i) and BZLF1 (ii) at 6 and 24 h post-chemical induction of EBV-harboring B cells Jijoye (A), Raji (B), and Daudi (C). (D) Real-time RT-qPCR quantification of EBV miR-BHRF1-2-3p in spent media of non-induced and 24 h post-induction Jijoye (i), Raji (ii), and Daudi (iii) cells. (E–H) RT-dPCR quantification of cellular HHV-8 genes LANA and Rta at 6 and 24 h post-chemical induction of BC-1 (E) and BC-3 (G) cells. Real-time RT-qPCR quantification of HHV-8 miR-K12-10a-3p (i), miR-K12-10b (ii), and miR-K12-12-3p (iii) from spent media of non-induced and 24 h post-induction BC-1 (F) and BC-3 (H) cells. Data shown are from 4 biological repeats. Error bars represent the standard error of the mean.

Article Snippet: EBV type 2 was produced by treating the Jijoye cell line (ATCC CCL-87) with 4 mM sodium butyrate and 25 ng/mL TPA and concentrated by ultracentrifugation.

Techniques: Quantitative RT-PCR

Journal: Cancer treatment and research communications

Article Title: Exposure to a mycovirus containing Aspergillus Flavus reproduces acute lymphoblastic leukemia cell surface and genetic markers in cells from patients in remission and not controls.

doi: 10.1016/j.ctarc.2020.100279

Figure Lengend Snippet: Figure 3: Exposure of peripheral blood mononuclear cells (PBMC) from patients with acute lymphoblastic leukemia in remission / long term survivors(ALL) and sickle cell disease (SCD) to the culture media, plasma, avian leucosis virus, Mycocladus Corymbifera, aflatoxin, supernatant of CRL-2312 cell line culture, supernatant of culture of Aspergillus Flavus obtained from a home of a patient with acute lymphoblastic leukemia, Supernatant of CCL87 culture which contains EBV, supernatant of culture of Aspergillus Flavus co-cultured with purified Epstein Barr Virus (EBV) and purified EBV alone. Samples were analyzed for cell surface phenotype using flow cytometry every 24 hours for four days. Each day, samples were tested for CD10/CD19, CD34/CD19 and CD34/ CD117. Results were recorded as percentage of control. Aflatoxin indiscriminately induced abnormal cell surface phenotypes, both, in PBMC of ALL patients as well those of sickle cell disease controls. Supernatant of culture of Aspergillus Flavus, with and without EBV, and CCL87 or EBV alone induced positive cell surface

Article Snippet: Another source of EBV was Type 2 Burkitt‟s lymphoma Jijoye cell line (CCL87) (American Type Culture Collection (ATCC), Monassas, VA).

Techniques: Clinical Proteomics, Virus, Cell Culture, Purification, Flow Cytometry, Control

Journal: Journal of Virology

Article Title: CD21 (Complement Receptor 2) Is the Receptor for Epstein-Barr Virus Entry into T Cells

doi: 10.1128/JVI.00428-20

Figure Lengend Snippet: Comparison of gp350 amino acid sequences. The amino acid sequence (amino acids 1 to 504) of the gp350 protein of the EBV-1 strain Akata is shown. The known CD21 binding regions are underlined. The amino acid differences in gp350 of the EBV-2 strain Jijoye are in bold above the EBV-1 sequence.

Article Snippet: The EBV-2-positive Burkitt’s lymphoma cell line Jijoye (P-2003, P-3J; ATCC, Manassas, VA) and the EBV-negative cell line BL41 (gift of J. Sixbey, St. Jude Children's Research Hospital) were maintained in complete RPMI at 37°C and 5% CO 2 .

Techniques: Comparison, Sequencing, Binding Assay